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Bioprocesses · Process Analytical Technology

Follow the process
as it happens.

Two complementary readings connect molecular composition and output gases to bioprocess monitoring.

Explore applicationsAccess materials
20
technical materials
2
online technologies
1
integrated vision
RAMANComposition
MSGases
O₂CO₂VOCs

Critical variables of the process

Variables monitored

Select a need. The page indicates the most adherent analytical route and filters the related materials.

Recommended routeRaman + MS online

Combine molecular composition and metabolic activity to broaden the understanding of the process.

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Two signs. A process reading.

Complementary technologies

No technique answers everything. The choice starts with the critical variable and the process matrix.

Bioreactor in Raman monitoring context
HORIBAProcess Raman
Inline probe / single-use
Real-time molecular signature

01 · MOLECULAR SIGNATURE

Follow the chemistry of culture in real time.

Chemometric models transform Raman spectra into estimates of metabolites and process attributes. The literature is strong in CHO culture, fermentation, perfusion and upstream.

  • Glucose, lactate and amino acids
  • Biomass and cellular concentration
  • Calibration transfer and models
  • Power control and consistency

Technical Note: the articles support the application Raman; the HORIBA configuration must be defined and validated for each process. The inline system announced with Fujifilm is in co-development.

IPI — InProcess InstrumentsMass spectrometry online
Online mass spectrometer IPI GAM 2000
O₂CO₂VOCs
GAM 2000 BioR · continuous off-gas analysis

02 · OFF-GAS MS

It converts the gas phase into metabolic information.

The GAM 2000 Bio R measures gases online in fermenters and bioreactors. With appropriate flows and corrections, the signs support respiratory calculations and reading of metabolic transitions.

  • O2, CO2 and additional gases
  • OUR, CER e RQ
  • VOCs and volatile by-products
  • Multiplexation of bioreactors

Decision map

The appropriate signal for each dimension

A practical reading of what each technology adds to the monitoring of the bioprocess.

TechnologyObservaTypical variablesApplication in bioprocesses
RamanMolecular compositionGlucose, lactate, amino acids and biomassCell culture monitoring, fermentation, infusion and PAT strategies
IPI GAM 2000 BioRGas phaseO2, CO2 and VOCs; OUR, CER and RQ by calculationContinuous off-gas analysis in fermenters and bioreactors

Technical library

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Articles, application notes, material from manufacturers and revisions — organized by technology.

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Given the decision

Monitoring your process

SENS can help combine technique, sampling, integration and analytical strategy for your bioprocess.

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